Yeast Vectors

For the Gateway SUS vectors please cite:

Grefen C, Obrdlik P and Harter K (2009) "The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS)." Methods Mol. Biol. 479:217-33.

For the SUS yeast strains please cite:

Obrdlik, et al. (2004) "K⁺ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions." PNAS 101:12242-7.

Grefen C, Lalonde S and Obrdlik P (2007) "Split-ubiquitin system for identifying protein-protein interactions in membrane and full-length proteins." Curr. Protoc. Neurosci. Chapter 5: Unit 5.27.

Available are:

Name	Map	Sequence
pMetYC-Dest	pMetYC-Dest.pdf	pMetYC-Dest.gb
pNX32-Dest	pNX32-Dest.pdf	pNX32-Dest.gb
pXN22-Dest	pXN22-Dest.pdf	pXN22-Dest.gb
pXNubA22-Dest	pXNubA22-Dest.pdf	pXNubA22-Dest.gb
pVTU-Dest	pVTU-Dest.pdf	pVTU-Dest.gb
pNubWt-Xgate (non-GW)	pNubWt-Xgate.pdf	pNubWt-Xgate.gb

Alternatively, you can download a zipped collection of all the above files here: SUS-Dest-vectors.zip

Sequence information is provided in genebank (*.gb) files; you can either open them directly with DNA software (VectorNTI, clc workbench, editseq) or simply use a text editor tool.

Feel free to contact Dr Christopher Grefen (c.grefen@bio.gla.ac.uk) for details on the SUS.