The Rhizo-II Root Biometrics Suite

The Rhizo-II Root Biometrics Suite

[The EZ-Root-VIS Pipeline]

The Rhizo-II Root Biometrics Suite is a software package for Windows platforms that includes both a new version of the popular EZ-Rhizo program and Root-VIS, an application for visualisation and analysis of root system architecture (RSA) data acquired by EZ-Rhizo.

The software is ideally suited for developmental and phenotypic descriptions of individual plants and plant species, accessions, and mutants, and of growth under varying nutritional and environmental conditions.  It facilitates new research perspectives in the areas of functional genomics, breeding and QTL analysis, and in predictive analysis of plant performance.

The two programs share a common graphical user interface (GUI) and data can easily be shared between them using databases built using XML.


The EZ-Rhizo application enables the analysis of images of roots grown on two-dimensional media such as agar plates.  The program provides a semi-automated system for root detection, measurement of multiple parameters relevant to the root architecture, data storage and data analysis.

The Root-VIS application provides rapid and informative visualisations of comparative averages extracted from RSA datasets acquired by the EZ-Rhizo program (and other software); the program also produces numerical output, in a choice of formats, which can be used as source data for more detailed analysis elsewhere.


You can download the software installers from either of the following links, depending on your operating system:

(If you’re not sure which type of Windows you have, then try the 64-bit installer first: this will not work if your platform is 32-bit! However, the 32-bit version will install on 64-bit platforms, but with lower overall performance.)  The files are Microsoft Installer Packages; either “Save” the relevant one to your local PC then double-click, or you can “Run” from the website (if your browser gives you the option). The software should work on any version of Windows from XP (Service Pack 3) onwards: Vista, 7, 8.1 and 10 (in ‘Classic Desktop’ mode). There is not (yet) a version for Mac OSX but the software will run under “Parallels” (Windows on Mac).

Terms of Use

The Rhizo-II Root Biometrics Suite is available free of charge for use in academic research institutions; however, registration/activation of the software is required.  When publishing data collected, or analyses made, using the software, please cite:

Zaigham Shahzad, Fabian Kellermeier, Emily M. Armstrong, Simon Rogers, Guillaume Lobet, Anna Amtmann and Adrian Hills: “EZ-Root-VIS – A Software Pipeline for the Visual Reconstruction of Averaged Root System Architecture”  Plant Physiology, 2018, In Press (DOI: 10.1104/pp.18.00217)