LABORATORY OF PLANT PHYSIOLOGY AND BIOPHYSICS

  • Begonia
    Begonias represent one of the largest and most
    diverse genera among the angiosperms. Several
    Begonia species exhibit complex stomatal
    patterning and variable stomatal clustering,
    which may be important for their physiological
    adaptation to extreme wet environments such as
    around waterfalls, and is a focus of research
    in the Laboratory.
  • Cyanobacteria
    Cyanobacteria, also known as blue-green algae,
    obtain their energy through photosynthesis,
    making use of much the same pathways as plants.
    It is thought that modern plants arose through
    a symbiotic association with cyanobacteria,
    which have since evolved to form the chloroplasts
    of higher plants thus providing energy to the
    host. For these reasons, cyanobacteria have long
    been used as a model with which to study the
    process of photosynthesis.
  • Broad Bean
    Vicia faba is familiar to us as the broad bean
    or butterbean. It is a robust, upright annual
    that grows well in cooler environments. It’s
    rounded, segmented leaves are easy to dissect
    by hand and have long been a favourite for
    research on stomatal guard cells and their
    control of gas exchange in plants. Data that
    comes from studies of Vicia remains the gold
    standard in the field of membrane transport
    and its control.
  • Water Scarcity
    Water scarcity and changes in the global
    environment are the most serious threats to
    global food security. Many parts of the USA,
    Australia and Asia as well as the mediterranean
    countries have seen substantial increases in
    water deficits over the past decade. Even in the
    UK, the demand for irrigation water has risen
    almost 10-fold in the past 20 years. Recurrence
    of the Dust Bowl phenomenon that devastated the
    American wheat belt is a real concern.
  • Arabidopsis Flowers
    The Arabidopsis genome was the first plant genome
    to be fully sequenced. This knowledge, together
    with a large body of mutational data, tools for
    genetic manipulation and access to closerelatives
    with varied physiological traits, greatly speeds
    fundamental research in the plant sciences.
  • Oilseed rape
    Brassica napus is a major focus
    for translational research building on work
    from Arabidopsis. The leaves and stems are
    commonly eaten in Southeast Asia and are often
    found in asian groceries sold as tender greens.
    Over 60% of its cultivation in the EU currently
    goes into biodiesel production. Rapeseed demands
    substantial water and N fertilization, although
    newer varieties grown in Canada have been
    reported to be more drought-tolerant.
  • Jungle Night
    Amorphophallus Paeoniifolius is one of a number
    of giant arums found in tropical central
    Americas. Its close relative, Amorphophallus
    titanium, produces the largest flower in the
    world, which can grow to over 2 meters in
    height. Like many giant arums, A. paeoniifolius
    grows from an elongated tuber. The leaf
    structure, stomatal organisation and venation,
    visible here, are well-adapted for highly humid
    environments.

Electrophysiology - Henry's EP Suite

Henry’s EP Suite was developed, from its inception, as full-featured, GUI-based software that operates on the Microsoft® Windows™ platform. The Suite is both extremely efficient and user-friendly. All of the offline utilities can be used with data collected and imported from other software packages.

Clamp control and data acquisition are driver-supported for compatibility with Scientific Solutions' LabMaster DMA and PCI-based LabMaster PRO boards as well as other ADC/DAC interfaces such as Instratech and Heka systems. The software is configurable for use with a wide range of amplifiers, including Heka systems, the Dagan PC-ONE system and Axon Instruments GeneClamp 500B and AxoClamp 2B.

For enquiries and further information about Henry's EP Suite, please e-mail henry@psrg.org.uk.

 

Download EP Suite setup program for 32-bit Windows (27 MB)

 

Download EP Suite setup program for 64-bit Windows (32 MB)

 

Download example files for synchronized image acquisition (45 MB)

 

Key Notes



Hardware Requirements

HARDWARE REQUIREMENTS

Pentium II or better CPU, running Microsoft Windows 2000, XP, Vista or Windows 7; Administrator rights to install the package.

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Included Features

INCLUDED FEATURES

*Voltage and current clamp control facilities, easy access for rapid and intuitive protocol editing, inbuilt audio monitor, real-time graphical chart recorder and clamp monitors, real-time steady-state current-voltage monitoring, sketchpad for runtime comparisons, and much more.

*Image and spectrographic acquisition utilities that synchronise with clamp control and data collection (requires the SIDX interface — available from the Bruxton Corporation — installed, and you must have one of the many supported CCD devices fitted).

*N-Pro, a ‘smart filter’ that improves signal resolution against background noise.

*Vicar, an online chart recorder.

*Pandora, an ever-increasing collection of utilities for analysis of single-channel (patch-clamp) data.

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Additional Information

ADDITIONAL INFORMATION

(1)Windows-98/ME platforms are no longer supported.

2) Henry’s EP Suite is built to run in Windows 64-Bit environment, but has been extensively tested only in the 32-bit environment. Therefore we advise that your use it in the 64-bit environment with caution. Please let us know of any problems you encounter, and we shall endeavour to correct them as expediently as possible.

3) If you have Henry II's EP Suite (software prior to V-3.0) installed, you should uninstall it before installing the latest version. Please also delete any Y-Science device drivers (use the Control Panel > System > Hardware > Device Manager in Windows). Before doing so, make note of the ADC Board/Rig setting. If you have an installation of Henry's EP Suite later than V-3.0, you can simply use the installation programs provided above to update your software and device drivers. The current release is V-3.5.5.5 (Spring, 2012).

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Terms of Use

TERMS OF USE

Henry's EP Suite is available free of charge and does not require a licence; however, when publishing data collected or analyses made using the software, please cite:

Blatt, M.R. (1987) Electrical Characteristics of Stomatal Guard Cells: The Contribution of ATP-Dependent “Electrogenic” Transport Revealed by Current-Voltage and Difference-Current-Voltage Analysis. J. Membrane Biol. 98:257-74.

Hills, A. and Volkov, V. (2004) Electrophysiology Equipment and Software. In: Membrane Transport in Plants, Blatt, M.R., Ed., Annual Plant Reviews, v. 15, pp. 40-71.

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ADC/DAC Board Drivers

ADC/DAC BOARD DRIVERS

The Y-Science ADC/DAC Board Drivers provide the means by which the component applications of Henry’s EP Suite communicate with and control the installed ADC/DAC hardware in your PC. They present an identical interface to the software, irrespective of the particular hardware model in use. These drivers are system components that adhere to the Windows Driver Model (wdm) scheme and are fully compatible the Windows XP, Vista and Windows 7 operation. They will not run under Windows 95/98/ME or Windows NT4.

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